Therapeutic potential of extracellular vesicles derived from cardiac progenitor cells in rodent models of chemotherapy-induced cardiomyopathy
نویسندگان
چکیده
Background Current treatments of chemotherapy-induced cardiomyopathy (CCM) are limited efficacy. We assessed whether repeated intravenous injections human extracellular vesicles from cardiac progenitor cells (EV-CPC) could represent a new therapeutic option and EV manufacturing according to Good Manufacturing Practices (GMP)-compatible process did not impair their bioactivity. Methods Immuno-competent mice received intra-peritoneal (IP) doxorubicin (DOX) (4 mg/kg each; cumulative dose: 12 mg/kg) were then intravenously (IV) injected three times with EV-CPC (total 30 billion). Cardiac function was 9–11 weeks later by magnetic resonance imaging (CMR) using strain as the primary end point. Then, immuno-competent rats 5 IP DOX (3 dose 15 followed 3 equal IV GMP-EV 100 two dimensional-echocardiography. Results In chronic mouse model CCM, + placebo-injected hearts incurred significant decline in basal (global, epi- endocardial) circumferential compared sham DOX-untreated ( p = 0.043, 0.042, 0.048 respectively) while preserved these indices. Global longitudinal similar pattern. rat model, also left ventricular end-systolic end-diastolic volumes untreated controls. Conclusions Intravenously-injected derived CPC have cardio-protective effects which may make them an attractive user-friendly for treatment CCM.
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ژورنال
عنوان ژورنال: Frontiers in Cardiovascular Medicine
سال: 2023
ISSN: ['2297-055X']
DOI: https://doi.org/10.3389/fcvm.2023.1206279